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Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

Author:
  • Diane M Rutherford
  • Mogens P Nielsen
  • Susanne K Hansen
  • Michael-Robin Witt
  • Ola Bergendorff
  • Olov Sterner
Publishing year: 1992
Language: English
Pages: 224-226
Publication/Series: Neuroscience Letters
Volume: 135
Issue: 2
Document type: Journal article
Publisher: Elsevier

Abstract english

Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.

Keywords

  • Neurosciences
  • γ-Aminobutyric acid
  • Benzodiazepine receptor
  • [35S]TBPS binding
  • Salvia officinalis
  • Diterpenoid
  • Carnosol
  • Carnosic acid

Other

Published
  • ISSN: 0304-3940
Olov Sterner
E-mail: olov.sterner [at] science.lu.se

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