Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Epitope-specificity of recombinant antibodies reveals promiscuous peptide-binding properties.

Author:
  • Niclas Olsson
  • Stefan Wallin
  • Peter James
  • Carl Borrebaeck
  • Christer Wingren
Publishing year: 2012
Language: English
Pages: 1897-1910
Publication/Series: Protein Science
Volume: 21
Issue: 12
Document type: Journal article
Publisher: The Protein Society

Abstract english

Protein-peptide interactions are a common occurrence and essential for numerous cellular processes, and frequently explored in broad applications within biology, medicine, and proteomics. Therefore, understanding the molecular mechanism(s) of protein-peptide recognition, specificity, and binding interactions will be essential. In this study, we report the first detailed analysis of antibody-peptide interaction characteristics, by combining large-scale experimental peptide binding data with the structural analysis of eight human recombinant antibodies and numerous peptides, targeting tryptic mammalian and eukaryote proteomes. The results consistently revealed that promiscuous peptide-binding interactions, that is, both specific and degenerate binding, were exhibited by all antibodies, and the discovery was corroborated by orthogonal data, indicating that this might be a general phenomenon for low-affinity antibody-peptide interactions. The molecular mechanism for the degenerate peptide-binding specificity appeared to be executed through the use of 2-3 semi-conserved anchor residues in the C-terminal part of the peptides, in analogue to the mechanism utilized by the major histocompatibility complex-peptide complexes. In the long-term, this knowledge will be instrumental for advancing our fundamental understanding of protein-peptide interactions, as well as for designing, generating, and applying peptide specific antibodies, or peptide-binding proteins in general, in various biotechnical and medical applications.

Keywords

  • Immunology in the medical area
  • antibody specificity
  • anti-peptide antibody
  • peptide binding
  • immunoaffinity peptide capture
  • mass spectrometry

Other

Published
  • CREATE Health
  • ISSN: 1469-896X
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

90

Director

Create Health

+46 46 222 96 13

90