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CD4+ T-cells have a key instructive role in educating dendritic cells in allergy

Author:
  • Kristina Larsson
  • Malin Lindstedt
  • Kristina Lundberg
  • Linda Dexlin Mellby
  • Christer Wingren
  • Magnus Korsgren
  • Lennart Greiff
  • Carl Borrebaeck
Publishing year: 2009
Language: English
Pages: 1-15
Publication/Series: International Archives of Allergy and Immunology
Volume: 149
Issue: 1
Document type: Journal article
Publisher: Karger

Abstract english

Background: Dendritic cells (DCs) are central in allergy as regulators of the Th1/Th2 balance. We have recently demonstrated a unique transcriptional profile of DCs in patients with ongoing allergy compared with healthy subjects and shown that crosstalk between DCs and memory T cells affects the transcriptional profile of T cells. However, the transcriptional profile of DCs educated by T cells in allergy is unknown. Methods: In the present study, we have examined the transcriptional profiles of DCs after stimulation with grass pollen allergens, Phleum pratense and coculture with autologous CD4+ memory T cells using high-density microarray. Protein analysis was performed using flow cytometry and recombinant antibody protein microarrays. Patients with allergic rhinitis and healthy subjects were compared. Results: The results reveal a distinct T-cell-induced DC profile in atopic individuals. Accordingly, about 170 genes were upregulated and 40 genes downregulated. For example, the chemokine receptor CXCR4 and the tumor necrosis factor receptor CD30 were upregulated in DCs derived from atopic donors, and this could also be verified at the protein level. Conclusion: We conclude that crosstalk between CD4+ memory T cells and autologous DCs induces transcriptional reprogramming in DCs. This finding suggests that T cells have a key instructive role in educating DCs in Th2-type responses.

Keywords

  • Respiratory Medicine and Allergy

Other

Published
  • ISSN: 1423-0097
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

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