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Carl Borrebaeck
Professor
![Carl Borrebaeck](/sites/madforcancer.lu.se/files/styles/lu_personal_page_desktop/public/carlborrebaeck2012_0.jpg.webp?itok=Kthok9cj)
CD4+ T-cells have a key instructive role in educating dendritic cells in allergy
Author
Summary, in English
Background: Dendritic cells (DCs) are central in allergy as regulators of the Th1/Th2 balance. We have recently demonstrated a unique transcriptional profile of DCs in patients with ongoing allergy compared with healthy subjects and shown that crosstalk between DCs and memory T cells affects the transcriptional profile of T cells. However, the transcriptional profile of DCs educated by T cells in allergy is unknown. Methods: In the present study, we have examined the transcriptional profiles of DCs after stimulation with grass pollen allergens, Phleum pratense and coculture with autologous CD4+ memory T cells using high-density microarray. Protein analysis was performed using flow cytometry and recombinant antibody protein microarrays. Patients with allergic rhinitis and healthy subjects were compared. Results: The results reveal a distinct T-cell-induced DC profile in atopic individuals. Accordingly, about 170 genes were upregulated and 40 genes downregulated. For example, the chemokine receptor CXCR4 and the tumor necrosis factor receptor CD30 were upregulated in DCs derived from atopic donors, and this could also be verified at the protein level. Conclusion: We conclude that crosstalk between CD4+ memory T cells and autologous DCs induces transcriptional reprogramming in DCs. This finding suggests that T cells have a key instructive role in educating DCs in Th2-type responses.
Department/s
- Department of Immunotechnology
- Division of Clinical Chemistry and Pharmacology
- Otorhinolaryngology (Lund)
Publishing year
2009
Language
English
Pages
1-15
Publication/Series
International Archives of Allergy and Immunology
Volume
149
Issue
1
Document type
Journal article
Publisher
Karger
Topic
- Respiratory Medicine and Allergy
Status
Published
ISBN/ISSN/Other
- ISSN: 1423-0097