Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Tissue proteome profiling of preeclamptic placenta using recombinant antibody microarrays

Author:
  • Linda Dexlin Mellby
  • Anna Sandström Gerdtsson
  • Magnus Centlow
  • Sara Sjögren
  • Stefan Hansson
  • Carl Borrebaeck
  • Christer Wingren
Publishing year: 2010
Language: English
Pages: 794-807
Publication/Series: Proteomics Clinical Applications
Volume: 4
Issue: 10-11
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

Purpose: Preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE. Experimental design: We used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions. Results: The data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values <= 0.91) could be distinguished. Notably, the data implied that all subgroups, but preeclampsia with bilateral notching and IUGR, could be further classified into novel subsets (ROC AUC values of 1.0) displaying different inflammatory signatures. Conclusions and clinical relevance: We have taken one step further toward de-convoluting the complex features of PE at the molecular level using affinity proteomics.

Keywords

  • Obstetrics, Gynecology and Reproductive Medicine
  • Preeclampsia
  • Membrane proteomics
  • Affinity proteomics
  • Antibody microarrays
  • Protein expression profiling

Other

Published
  • CREATE Health
  • ISSN: 1862-8354
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

90

Director

Create Health

+46 46 222 96 13

90