The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Carl Borrebaeck

Carl Borrebaeck

Professor

Carl Borrebaeck

Tissue proteome profiling of preeclamptic placenta using recombinant antibody microarrays

Author

  • Linda Dexlin Mellby
  • Anna Sandström Gerdtsson
  • Magnus Centlow
  • Sara Sjögren
  • Stefan Hansson
  • Carl Borrebaeck
  • Christer Wingren

Summary, in English

PURPOSE: preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE.

EXPERIMENTAL DESIGN: we used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions.

RESULTS: the data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values ≤ 0.91) could be distinguished. Notably, the data implied that all subgroups, but preeclampsia with bilateral notching and IUGR, could be further classified into novel subsets (ROC AUC values of 1.0) displaying different inflammatory signatures.

CONCLUSIONS AND CLINICAL RELEVANCE: we have taken one step further toward de-convoluting the complex features of PE at the molecular level using affinity proteomics.

Department/s

  • Department of Immunotechnology
  • Obstetrics and Gynaecology (Lund)

Publishing year

2010

Language

English

Pages

794-807

Publication/Series

Proteomics Clinical Applications

Volume

4

Issue

10-11

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Obstetrics, Gynecology and Reproductive Medicine

Keywords

  • Adult
  • Female
  • Gene Expression Profiling/methods
  • Gestational Age
  • Humans
  • Middle Aged
  • Placenta/metabolism
  • Pre-Eclampsia/genetics
  • Pregnancy
  • Protein Array Analysis/methods
  • Proteome/analysis
  • Recombinant Proteins/genetics
  • Young Adult

Status

Published

ISBN/ISSN/Other

  • ISSN: 1862-8354