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Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reversed genomics

Author:
  • Johan Fransson
  • Sara Ek
  • Peter Ellmark
  • Eskil Söderlind
  • Carl Borrebaeck
  • Christina Furebring
Publishing year: 2004
Language: English
Pages: 235-242
Publication/Series: Cancer Letters
Volume: 208
Issue: 2
Document type: Journal article
Publisher: Elsevier

Abstract english

Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our strategy to provide a unique set of human antibodies, potentially useful in immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved.

Keywords

  • Cancer and Oncology

Other

Published
  • ISSN: 1872-7980
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

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