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Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.

Author:
  • Linda Dexlin Mellby
  • Anna Sandström Gerdtsson
  • Linn Antberg
  • Jenny Gunnarsson
  • Stefan Hansson
  • Carl Borrebaeck
  • Christer Wingren
Publishing year: 2011
Language: English
Pages: 1550-1554
Publication/Series: Proteomics
Volume: 11
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.

Keywords

  • Obstetrics, Gynecology and Reproductive Medicine

Other

Published
  • CREATE Health
  • ISSN: 1615-9861
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

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Director

Create Health

+46 46 222 96 13

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