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antibody microarray analysis of directly labelled proteomes

Author:
  • Christer Wingren
  • Carl Borrebaeck
Publishing year: 2008
Language: English
Pages: 55-61
Publication/Series: Current Opinion in Biotechnology
Volume: 19
Issue: 1
Document type: Journal article review
Publisher: Elsevier

Abstract english

In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic standards for performing the antibody microarray data analysis have emerged, though general standard operating procedure(s) remains to be implemented.

Keywords

  • Immunology in the medical area

Other

Published
  • CREATE Health
  • ISSN: 0958-1669
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

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Director

Create Health

+46 46 222 96 13

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