Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing

Summary, in English

The composition of serum proteins is reflecting the current health status and can, with the right tools, be used to detect early signs of disease, such as an emerging cancer. An earlier diagnosis of cancer would greatly increase the chance of an improved outcome for the patients. However, there is still an unmet need for proficient tools to decipher the information in the blood proteome, which calls for further technological development. Here, we present a proof-of-concept study that demonstrates an alternative approach for multiplexed protein profiling of serum samples in solution, using DNA barcoded scFv antibody fragments and next generation sequencing. The outcome shows high accuracy when discriminating samples derived from pancreatic cancer patients and healthy controls and represents a scalable alternative for serum analysis.

• Author: Mattias Brofelth; Anna Isinger Ekstrand; Shashank Gour; Ronnie Jansson; My Hedhammar; Björn Elleby; Anders Kvist; Christer Wingren; Ulrika Axelsson; Carl A.K. Borrebaeck
• Department/s: LUCC: Lund University Cancer Centre; Department of Immunotechnology; Create Health; Familial Breast Cancer
• Publishing year: 2020
• Language: English
• Publication/Series: Communications Biology
• Volume: 3
• Issue: 1
• Links: http://dx.doi.org/10.1038/s42003-020-1068-0
• Document type: Journal article
• Publisher: Nature Publishing Group
• Topic: Cell and Molecular Biology; Cancer and Oncology
• Status: Published
• Research group: Familial Breast Cancer
• ISBN/ISSN/Other: ISSN: 2399-3642