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Designing proteins to crystallize through beta-strand pairing

Author:
  • Christer Wingren
  • AB Edmundson
  • Carl Borrebaeck
Publishing year: 2003
Language: English
Pages: 255-264
Publication/Series: Protein Engineering
Volume: 16
Issue: 4
Document type: Journal article
Publisher: Oxford University Press

Abstract english

Inherent difficulties in growing protein crystals are major concerns within structural biology and particularly in structural proteomics. Here, we describe a novel approach of engineering target proteins by surface mutagenesis to increase the odds of crystallizing the molecules. To this end, we have exploited our recent triad-hypothesis using proteins with crystallographically defined beta-structures as the principal models. Crystal packing analyses of 182 protein structures belonging to 21 different superfamilies implied that the propensities to crystallize could be engineered into target proteins by replacing short segments, 5-6 residues, of their beta-strands with 'cassettes' of suitable packing motifs. These packing motifs will generate specific crystal packing interactions that promote crystallization. Key features of the primary and tertiary structures of such packing motifs have been identified for immunoglobulins. Further, packing motifs have been engineered successfully into six model antibodies without disturbing their capabilities to be produced, their immunoreactivity and their overall structure. Preliminary crystallization analyses have also been performed. Taken together, the procedures outline a rational protocol for crystallizing proteins by surface mutagenesis. The importance of these findings is discussed in relation to the crystallization of proteins in general.

Keywords

  • Immunology in the medical area
  • structural proteomics
  • protein crystallization
  • crystal engineering
  • directed crystallization
  • triad-hypothesis

Other

Published
  • ISSN: 1460-213X
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

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