The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Carl B

Carl Borrebaeck

Professor

Carl B

New approach to steroid separation based on a low affinity IgM antibody

Author

  • Magnus Strandh
  • Mats Ohlin
  • Carl A K Borrebaeck
  • Sten Ohlson

Summary, in English

IgM antibodies are often of low affinity (dissociation constant (K(d)) > 10-5 M) and therefore they are usually neglected as tools in, e.g., immunoassays. Previous studies have shown that low affinity biological interactions can be studied and exploited in affinity chromatography, biosensor technology and capillary electrophoresis. In this study we have demonstrated that IgM can be a useful ligand for analytical separation of antigens in weak affinity chromatography (WAC). A low affinity human monoclonal IgM antibody, directed at digoxin, was produced in a hybridoma cell culture, purified to homogeneity and immobilized onto an HPLC support. The IgM HPLC column displayed specific weak affinity retention in the 0.01- 0.1 mM range as evaluated with digoxin and ouabain. The specificity was not affected when samples of ouabain in a crude environment of diluted serum were separated on the IgM column. These findings suggest an approach in immunoadsorbent technology where biomolecules can be analyzed and separated with weak affinity chromatography using IgM as a general affinity ligand.

Department/s

  • Department of Immunotechnology

Publishing year

1998-05-01

Language

English

Pages

73-79

Publication/Series

Journal of Immunological Methods

Volume

214

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Keywords

  • Digoxin
  • Low affinity monoclonal IgM antibody
  • Weak affinity chromatography (WAC)

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-1759