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Affinity and Epitope Profiling of Mouse Anti-CD40 Monoclonal Antibodies

Author:
  • Ann-Christin Malmborg Hager
  • Peter Ellmark
  • Carl Borrebaeck
  • Christina Furebring
Publishing year: 2003
Language: English
Pages: 517-524
Publication/Series: Scandinavian Journal of Immunology
Volume: 57
Issue: 6
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

The CD40-CD40L interaction plays a critical role in both humoral and cellular immune responses and interfering antibodies have been suggested as an effective approach for the treatment of lymphomas and autoimmune diseases. In this study we have profiled a panel of mouse antihuman CD40 monoclonal antibodies (MoAbs), regarding their CD40 binding affinity and epitope-specificity relative to the CD40L binding in relation to their cellular activating potential. Despite a rather similar domain-recognition profile, the MoAbs blocked the CD40L binding to a varying degree, with MoAb 5C3 being the poorest inhibitor. There was no correlation between affinity and cellular activation potential. In contrast, a correlation between the ability to block CD40L-binding and activation potential could be seen. We believe that this analysis of several mouse anti-CD40 antibodies can be used to develop strategies for producing new human anti-CD40 antibodies that can more effectively induce or block B-cell proliferation.

Keywords

  • Immunology in the medical area

Other

Published
  • ISSN: 1365-3083
Carl B
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

Professor

Department of Immunotechnology

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