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Carl B

Carl Borrebaeck

Professor

Carl B

Effect of cell-derived growth factors and cytokines on the clonal outgrowth of EBV-infected B cells and established lymphoblastoid cell lines

Author

  • P. Ifversen
  • Z. Xiu-Mei
  • M. Ohlin
  • J. Zeuthen
  • C. A K Borrebaeck

Summary, in English

Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and is widely used as a tool for the establishment of B cell lines producing human monoclonal antibodies. However, because of low transformability, low clonability, and the inherent instability of EBV-infected B cells, valuable antibody-producing B cells are often lost during this procedure. We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments, whereas monocyte-derived factors and monocyte cell lines were less capable than peripheral blood mononuclear cells in enhancing cellular outgrowth and cloning. Furthermore, the human T cell hybridoma cell line MP6 that secretes a B cell growth and differentiation factor, recently identified as an isoform of thioredoxin, is also capable of stimulating EBV-infected B cells and lymphoblastoid cell lines. Co-cultivation of EBV-infected B cells with MP6 cells significantly enhanced the cloning efficiency at the 1 cell/well level. The present results also suggest that one potential role of the MP6-derived thioredoxin could be the up regulation of IL-6 receptor expression in EBV-infected B cells.

Department/s

  • Department of Immunotechnology

Publishing year

1993

Language

English

Pages

115-123

Publication/Series

Human Antibodies and Hybridomas

Volume

4

Issue

3

Document type

Journal article

Publisher

Butterworth Scientific Ltd.

Keywords

  • B cells
  • EBV infection
  • MP6
  • Thioredoxin

Status

Published

ISBN/ISSN/Other

  • ISSN: 0956-960X