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Sensitivity of HIV-1 primary isolates to human anti-CD40 antibody-mediated suppression is related to co-receptor use

Author:
  • Sisay A Abayneh
  • Peter Ellmark
  • Ulf Karlsson
  • Henrik Andersson
  • Carl Borrebaeck
  • Ingrid Karlsson
  • Eva Maria Fenyö
Publishing year: 2008
Language: English
Pages: 447-452
Publication/Series: AIDS Research and Human Retroviruses
Volume: 24
Issue: 3
Document type: Journal article
Publisher: Mary Ann Liebert, Inc.

Abstract english

The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric receptors. In comparison, HIV-1 isolates with broader use of CCR5/CXCR4 chimeric receptors or with CXCR4 use were less sensitive to anti-CD40-induced suppression. The results indicate that HIV-1 replication is inhibited by human anti-CD40 monoclonal antibodies through the mechanism of CC-chemokine induction. This effect is thus restricted to HIV-1 isolates sensitive to inhibition by CC-chemokines.

Keywords

  • Pharmacology and Toxicology
  • Microbiology in the medical area

Other

Published
  • Drug Target Discovery
  • ISSN: 1931-8405
Carl B
Carl Borrebaeck
E-mail: carl.borrebaeck [at] immun.lth.se

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Department of Immunotechnology

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