The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Carl B

Carl Borrebaeck

Professor

Carl B

A community standard format for the representation of protein affinity reagents

Author

  • David E. Gloriam
  • Sandra Orchard
  • Daniela Bertinetti
  • Erik Bjorling
  • Erik Bongcam-Rudloff
  • Carl Borrebaeck
  • Julie Bourbeillon
  • Andrew R. M. Bradbury
  • Antoine de Daruvar
  • Stefan Duebel
  • Ronald Frank
  • Toby J. Gibson
  • Larry Gold
  • Niall Haslam
  • Friedrich W. Herberg
  • Tara Hiltke
  • Joerg D. Hoheisel
  • Samuel Kerrien
  • Manfred Koegl
  • Zoltan Konthur
  • Bernhard Korn
  • Ulf Landegren
  • Luisa Montecchi-Palazzi
  • Sandrine Palcy
  • Henry Rodriguez
  • Sonja Schweinsberg
  • Volker Sievert
  • Oda Stoevesandt
  • Michael J. Taussig
  • Marius Ueffing
  • Mathias Uhlen
  • Silvere van der Maarel
  • Christer Wingren
  • Peter Woollard
  • David J. Sherman
  • Henning Hermjakob
Show all

Summary, in English

Protein affinity reagents (PARs), most commonly antibodies, are essential reagents for protein characterization in basic research, biotechnology, and diagnostics as well as the fastest growing class of therapeutics. Large numbers of PARs are available commercially; however, their quality is often uncertain. In addition, currently available PARs cover only a fraction of the human proteome, and their cost is prohibitive for proteome scale applications. This situation has triggered several initiatives involving large scale generation and validation of antibodies, for example the Swedish Human Protein Atlas and the German Antibody Factory. Antibodies targeting specific subproteomes are being pursued by members of Human Proteome Organisation (plasma and liver proteome projects) and the United States National Cancer Institute (cancer-associated antigens). ProteomeBinders, a European consortium, aims to set up a resource of consistently quality-controlled protein-binding reagents for the whole human proteome. An ultimate PAR database resource would allow consumers to visit one online warehouse and find all available affinity reagents from different providers together with documentation that facilitates easy comparison of their cost and quality. However, in contrast to, for example, nucleotide databases among which data are synchronized between the major data providers, current PAR producers, quality control centers, and commercial companies all use incompatible formats, hindering data exchange. Here we propose Proteomics Standards Initiative (PSI)-PAR as a global community standard format for the representation and exchange of protein affinity reagent data. The PSI-PAR format is maintained by the Human Proteome Organisation PSI and was developed within the context of ProteomeBinders by building on a mature proteomics standard format, PSI-molecular interaction, which is a widely accepted and established community standard for molecular interaction data. Further information and documentation are available on the PSI-PAR web site. Molecular & Cellular Proteomics 9: 1-10, 2010.

Department/s

  • Department of Immunotechnology
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation

Publishing year

2010

Language

English

Pages

1-10

Publication/Series

Molecular & Cellular Proteomics

Volume

9

Issue

1

Document type

Journal article

Publisher

American Society for Biochemistry and Molecular Biology

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-9484