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Carl B

Carl Borrebaeck

Professor

Carl B

Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework

Author

  • P. Jirholt
  • M. Ohlin
  • C. A K Borrebaeck
  • E. Söderlind

Summary, in English

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

Department/s

  • Department of Immunotechnology

Publishing year

1998-07-30

Language

English

Pages

471-476

Publication/Series

Gene

Volume

215

Issue

2

Document type

Journal article

Publisher

Elsevier

Keywords

  • CDR shuffling
  • Gene assembly
  • Immunoglobulin germline gene
  • Primer design
  • Synthetic gene library

Status

Published

ISBN/ISSN/Other

  • ISSN: 0378-1119