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Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework

  • P. Jirholt
  • M. Ohlin
  • C. A K Borrebaeck
  • E. Söderlind
Publishing year: 1998-07-30
Language: English
Pages: 471-476
Publication/Series: Gene
Volume: 215
Issue: 2
Document type: Journal article
Publisher: Elsevier

Abstract english

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.


  • CDR shuffling
  • Gene assembly
  • Immunoglobulin germline gene
  • Primer design
  • Synthetic gene library


  • ISSN: 0378-1119
Carl B
Carl Borrebaeck
E-mail: carl.borrebaeck [at]


Department of Immunotechnology



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