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Genetic profiles of gastroesophageal cancer: combined analysis using expression array and tiling array-comparative genomic hybridization

Author:
  • Anna Isinger Ekstrand
  • Jan Johansson
  • Mattias Ohlsson
  • Princy Francis
  • Johan Staaf
  • Mats Jönsson
  • Åke Borg
  • Mef Nilbert
Publishing year: 2010
Language: English
Pages: 120-126
Publication/Series: Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00
Volume: 200
Issue: 2
Document type: Journal article
Publisher: Elsevier

Abstract english

We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were identified as putative target genes in cancers of the esophagus and the gastroesophageal junction, with upregulation in one quarter of the tumors. Gains/losses and gene expression profiles show strong similarity between cancers in the distal esophagus and the gastroesophageal junction with frequent upregulation of CDK6 and EGFR, whereas gastric cancer displays distinct genetic changes. These data suggest that molecular diagnostics and targeted therapies can be applied to adenocarcinomas of the distal esophagus and gastroesophageal junction alike. (C) 2010 Elsevier Inc. All rights reserved.

Keywords

  • Cancer and Oncology
  • Surgery
  • Esophageal Neoplasms: genetics
  • Oligonucleotide Array Sequence Analysis: methods
  • Comparative Genomic Hybridization: methods
  • Cyclin-Dependent Kinase 6: genetics
  • Receptor
  • Epidermal Growth Factor: genetics
  • Stomach Neoplasms: genetics

Other

Published
  • ISSN: 0165-4608
Åke Borg
Åke Borg
E-mail: ake.borg [at] med.lu.se

Principal investigator

Oncology and Pathology, MV

+46 46 275 25 52

MV 404 C21B2

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Project manager

Familial Breast Cancer

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Professor

Oncology and Pathology, MV

MV 404 C21C2

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