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Identification of new microRNAs in paired normal and tumor breast tissue suggests a dual role for the ERBB2/Her2 gene.

Author:
  • Helena Persson
  • Anders Kvist
  • Natalia Rego
  • Johan Staaf
  • Johan Vallon-Christersson
  • Lena Luts
  • Niklas Loman
  • Göran B Jönsson
  • Hugo Naya
  • Mattias Höglund
  • Åke Borg
  • Carlos Rovira
Publishing year: 2011
Language: English
Pages: 78-86
Publication/Series: Cancer Research
Volume: 71
Issue: 1
Document type: Journal article
Publisher: American Association for Cancer Research Inc.

Abstract english

To comprehensively characterize microRNA (miRNA) expression in breast cancer, we performed the first extensive next-generation sequencing expression analysis of this disease. We sequenced small RNA from tumors with paired samples of normal and tumor-adjacent breast tissue. Our results indicate that tumor identity is achieved mainly by variation in the expression levels of a common set of miRNAs rather than by tissue-specific expression. We also report 361 new, well-supported miRNA precursors. Nearly two-thirds of these new genes were detected in other human tissues and 49% of the miRNAs were found associated with Ago2 in MCF7 cells. Ten percent of the new miRNAs are located in regions with high-level genomic amplifications in breast cancer. A new miRNA is encoded within the ERBB2/Her2 gene and amplification of this gene leads to overexpression of the new miRNA, indicating that this potent oncogene and important clinical marker may have two different biological functions. In summary, our work substantially expands the number of known miRNAs and highlights the complexity of small RNA expression in breast cancer.

Keywords

  • Cancer and Oncology

Other

Published
  • CREATE Health
  • Pathology, Malmö
  • Cancer and non coding RNA
  • ISSN: 1538-7445
Åke Borg
Åke Borg
E-mail: ake.borg [at] med.lu.se

Principal investigator

Oncology and Pathology, MV

+46 46 275 25 52

MV 404 C21B2

90

Project manager

Familial Breast Cancer

90

Professor

Oncology and Pathology, MV

MV 404 C21C2

90