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Functional analysis of BRCA1 C-terminal missense mutations identified in breast and ovarian cancer families

Author:
  • J Vallon-Christersson
  • C Cayanan
  • K Haraldsson
  • N Loman
  • J T Bergthorsson
  • K Brøndum-Nielsen
  • A M Gerdes
  • P Møller
  • U Kristoffersson
  • Håkan Olsson
  • Åke Borg
  • A N Monteiro
Publishing year: 2001-02-15
Language: English
Pages: 60-353
Publication/Series: Human Molecular Genetics
Volume: 10
Issue: 4
Document type: Journal article
Publisher: Oxford University Press

Abstract english

Germline mutations in the breast and ovarian cancer susceptibility gene BRCA1 are responsible for the majority of cases involving hereditary breast and ovarian cancer. Whereas all truncating mutations are considered as functionally deleterious, most of the missense variants identified to date cannot be readily distinguished as either disease-associated mutations or benign polymorphisms. The C-terminal domain of BRCA1 displays an intrinsic transactivation activity, and mutations linked to disease predisposition have been shown to confer loss of such activity in yeast and mammalian cells. In an attempt to clarify the functional importance of the BRCA1 C-terminus as a transcription activator in cancer predisposition, we have characterized the effect of C-terminal germline variants identified in Scandinavian breast and ovarian cancer families. Missense variants A1669S, C1697R, R1699W, R1699Q, A1708E, S1715R and G1738E and a truncating mutation, W1837X, were characterized using yeast- and mammalian-based transcription assays. In addition, four additional missense variants (V1665M, D1692N, S1715N and D1733G) and one in-frame deletion (V1688del) were included in the study. Our findings demonstrate that transactivation activity may reflect a tumor-suppressing function of BRCA1 and further support the role of BRCA1 missense mutations in disease predisposition. We also report a discrepancy between results from yeast- and mammalian-based assays, indicating that it may not be possible to unambiguously characterize variants with the yeast assay alone. We show that transcription-based assays can aid in the characterization of deleterious mutations in the C-terminal part of BRCA1 and may form the basis of a functional assay.

Keywords

  • Medical Genetics
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • BRCA1 Protein
  • Breast Neoplasms
  • Cell Line
  • Databases, Factual
  • Dogs
  • Exons
  • Female
  • Genes, BRCA1
  • Genetic Variation
  • Humans
  • Mice
  • Middle Aged
  • Mutation, Missense
  • Ovarian Neoplasms
  • Pedigree
  • Promoter Regions, Genetic
  • Rats
  • Recombinant Fusion Proteins
  • Transcriptional Activation

Other

Published
  • Familial Breast Cancer
  • ISSN: 0964-6906
Åke Borg
Åke Borg
E-mail: ake.borg [at] med.lu.se

Principal investigator

Oncology and Pathology, MV

+46 46 275 25 52

MV 404 C21B2

90

Project manager

Familial Breast Cancer

90

Professor

Oncology and Pathology, MV

MV 404 C21C2

90