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Åke Borg

Åke Borg

Principal investigator

Åke Borg

Remarkable similarities of chromosomal rearrangements between primary human breast cancers and matched distant metastases as revealed by whole-genome sequencing.

Author

  • Man-Hung Eric Tang
  • Malin Dahlgren
  • Christian Brueffer
  • Tamara Tjitrowirjo
  • Christof Winter
  • Yilun Chen
  • Eleonor Olsson
  • Kun Wang
  • Therese Törngren
  • Martin Sjöström
  • Dorthe Grabau
  • Pär-Ola Bendahl
  • Lisa Rydén
  • Emma Niméus
  • Lao Saal
  • Åke Borg
  • Sofia Gruvberger

Summary, in English

To better understand and characterize chromosomal structural variation during breast cancer progression, we enumerated chromosomal rearrangements for 11 patients by performing low-coverage whole-genome sequencing of 11 primary breast tumors and their 13 matched distant metastases. The tumor genomes harbored a median of 85 (range 18-404) rearrangements per tumor, with a median of 82 (26-310) in primaries compared to 87 (18-404) in distant metastases. Concordance between paired tumors from the same patient was high with a median of 89% of rearrangements shared (range 61-100%), whereas little overlap was found when comparing all possible pairings of tumors from different patients (median 3%). The tumors exhibited diverse genomic patterns of rearrangements: some carried events distributed throughout the genome while others had events mostly within densely clustered chromothripsis-like foci at a few chromosomal locations. Irrespectively, the patterns were highly conserved between the primary tumor and metastases from the same patient. Rearrangements occurred more frequently in genic areas than expected by chance and among the genes affected there was significant enrichment for cancer-associated genes including disruption of TP53, RB1, PTEN, and ESR1, likely contributing to tumor development. Our findings are most consistent with chromosomal rearrangements being early events in breast cancer progression that remain stable during the development from primary tumor to distant metastasis.

Department/s

  • Breast cancer Proteogenomics
  • Breastcancer-genetics
  • Tumor microenvironment
  • Surgery (Lund)
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation

Publishing year

2015

Language

English

Pages

37169-37184

Publication/Series

Oncotarget

Volume

6

Issue

35

Document type

Journal article

Publisher

Impact Journals

Topic

  • Cancer and Oncology

Status

Published

Research group

  • Breast cancer Proteogenomics

ISBN/ISSN/Other

  • ISSN: 1949-2553