Åke Borg
Principal investigator
HER-2/neu amplification predicts poor survival in node-positive breast cancer
Author
Summary, in English
HER-2/neu protooncogene amplification and protein expression were analyzed with slot blot and Western blot techniques, respectively, in more than 300 invasive primary breast tumors of all stages. Amplification (2- greater than 30 copies) was found in 17% of these tumors and high expression was seen in 19%. There was a striking coincidence between gene amplification and high expression. Tumors associated with many involved axillary lymph nodes or with Stage IV disease were more often HER-2/neu amplified or overexpressed. Furthermore, gene alteration was strongly correlated with the absence of steroid receptors and with larger tumor size. High expression without gene amplification was seen in a minor subset of tumors of less aggressive character. Neither amplification nor overexpression was correlated with disease outcome for patients with negative axillary lymph nodes. For node-positive patients, however, HER-2/neu amplification was a significant predictor of early relapse and death (median follow-up = 45 months), and a similar trend, although not significant, existed for high gene expression. Multivariate analyses indicated that HER-2/neu alterations were not independent predictors of patient outcome.
Department/s
- Breastcancer-genetics
- Personalized Breast Cancer Treatment
- Tumor microenvironment
Publishing year
1990-07-15
Language
English
Pages
7-4332
Publication/Series
Cancer Research
Volume
50
Issue
14
Document type
Journal article
Publisher
American Association for Cancer Research Inc.
Topic
- Cancer and Oncology
Keywords
- Amino Acid Sequence
- Biomarkers, Tumor/analysis
- Blotting, Western
- Breast Neoplasms/analysis
- Female
- Gene Amplification
- Gene Expression
- Humans
- Immune Sera
- Lymphatic Metastasis
- Menopause
- Molecular Sequence Data
- Peptides/chemical synthesis
- Prognosis
- Protein-Tyrosine Kinases/genetics
- Proto-Oncogene Proteins/analysis
- Proto-Oncogenes
- Receptor, ErbB-2
- Receptors, Estrogen/analysis
- Receptors, Progesterone
Status
Published
Research group
- Personalized Breast Cancer Treatment
ISBN/ISSN/Other
- ISSN: 0008-5472