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Åke Borg

Åke Borg

Principal investigator

Åke Borg

Myoepithelium assessment with p63 immunostaining in formalinfixed paraffin-embedded breast cancer tissue pre-treated with RNA-later

Author

  • Anna Ehinger
  • Inger Remse
  • Kristina Lövgren
  • Cecilia Hegardt
  • Jari Häkkinen
  • Lao Saal
  • Johan Vallon-Christersson
  • Åke Borg

Summary, in English

Objective: To assessmyoepithelium with p63 in fresh breast cancer (BC)
tissue samples collected in RNA later for further analysis with Next
Generation Sequencing (NGS) technique. For a better understanding of
the NGS bulk-analysis, a central part of the sample in RNA-later is
formalin-fixed paraffin-embedded to score relative cellularity in % on
hematoxylin-eosin (HE) staining (% of invasive cancer, cancer in situ,
benign epithelium, lymphocytes and fat). Our aim is hence to test p63
immunohistochemistry (IHC) to highlight myoepithelium and to facilitate
the evaluation of the relative cellularity on BC-tissue pre-treated with
RNA-later.
Method: Two-hundred and twenty-four selected samples of fresh BC
tissue collected in RNA-later. A 10 mg central piece from each sample
was FFPE and assembled in a tissue-microarray (TMA) and sectioned to
HE and p63 IHC.
Results: All samples (n = 224) had internal control for myoepithelium
surrounding in situ cancer or benign epithelium. p63 showed positive
nuclear staining in myoepithelial cells in 92 % (206/224) of samples
and false negative p63 staining in 8 % (18/224).
Conclusion: p63 IHC is assessable in samples of FFPE BC-tissue pretreated
with RNA-later.

Department/s

  • Breastcancer-genetics
  • Personalized Breast Cancer Treatment
  • The Liquid Biopsy and Tumor Progression in Breast Cancer
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
  • Translational Oncogenomics
  • Familial Breast Cancer

Publishing year

2017-09-02

Language

English

Pages

299-299

Publication/Series

Virchows Archiv

Volume

471

Issue

Supplement 1

Document type

Conference paper: abstract

Publisher

Springer

Topic

  • Cancer and Oncology

Conference name

29th European Congress of Pathology

Conference date

2017-09-02

Conference place

Amsterdam, Netherlands

Status

Published

Project

  • Sweden Cancerome Analysis Network - Breast (SCAN-B): a large-scale multicenter infrastructure towards implementation of breast cancer genomic analyses in the clinical routine

Research group

  • Personalized Breast Cancer Treatment
  • The Liquid Biopsy and Tumor Progression in Breast Cancer
  • Translational Oncogenomics
  • Familial Breast Cancer

ISBN/ISSN/Other

  • ISSN: 1432-2307