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Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer

Author:
  • N N Nupponen
  • K Porkka
  • L Kakkola
  • Minna Tanner
  • Karin Persson
  • Åke Borg
  • Jorma Isola
  • T Visakorpi
Publishing year: 1999
Language: English
Pages: 1777-1783
Publication/Series: American Journal of Pathology
Volume: 154
Issue: 6
Document type: Journal article
Publisher: American Society for Investigative Pathology

Abstract english

Amplification at the long arm of chromosome 8 occurs in a large fraction of breast and prostate cancers. To clone the target genes for this amplification, we used suppression subtraction hybridization to identify overexpressed genes in the breast cancer cell line SK-Br-3, which harbors amplification at 8q (8q21 and 8q23-q24). A differentially expressed gene identified by SSH, the p40 subunit of eukaryotic translation initiation factor 3 (eIF3), was localized to 8q23 and found to be highly amplified and overexpressed in the breast and prostate cancer cell lines studied. High-level amplification of eIF3-p40 was found in 30% of hormone-refractory prostate tumors and in 18% of untreated primary breast tumors. In the vast majority of the cases, p40 and c-myc were amplified with equal copy numbers. Tumors with higher copy numbers of p40 than c-myc were also found. Expression of p40 mRNA was analyzed with in situ hybridization. The amplification of eIF3-p40 gene was associated with overexpression of its mRNA, as expected for a functional target gene of the amplification. These results imply that genomic aberrations of translation initiation factors, such as eIF3-p40, may contribute to the pathogenesis of breast and prostate cancer.

Keywords

  • Cell and Molecular Biology

Other

Published
  • ISSN: 1525-2191
Åke Borg
Åke Borg
E-mail: ake.borg [at] med.lu.se

Principal investigator

Oncology and Pathology, MV

+46 46 275 25 52

MV 404 C21B2

90

Project manager

Familial Breast Cancer

90

Professor

Oncology and Pathology, MV

MV 404 C21C2

90